pfa pbs (Thermo Fisher)
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pfa pbs
Pfa Pbs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pfa+pbs/Paraformaldehyde+Solution%2C+4%25+in+PBS/bio_rxiv__64898__2026__06__14__732116-294-13-14
Average 99 stars, based on 1 article reviews
Pfa Pbs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pfa+pbs/Paraformaldehyde+Solution%2C+4%25+in+PBS/bio_rxiv__64898__2026__06__14__732116-294-13-14
Average 99 stars, based on 1 article reviews
pfa pbs - by Bioz Stars,
2026-09
99/100 stars
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Expressing:Article Title: Stromal Prostaglandin is a Dominant Spatial Regulator of Cell-fate Plasticity in Colorectal Cancer Article Snippet: Once enough cells acquired, CAFs were harvested and washed in FACS buffer (PBS supplemented with 1 mmol/L EDTA + (Sigma, 03690), 25 mmol/L HEPES, and 1% FBS) before sorting for GFP expression through a BD FACSAria III. .. Cas9 expression was then assessed by harvesting and fixing the CAFs in 4% Clinical Proteomics:Article Title: Implantation of human mesenchymal stromal cell (MSC)-pellet for therapeutic angiogenesis Article Snippet: .. Plasma was obtained by centrifuging whole blood at 2000 g at 4°C for 15 min. Histological analysis After euthanizing the mouse, tissues and organs were harvested, fixed with 2% Staining:Article Title: Atomistic TCR-ligand interactions instruct memory T-cell differentiation Article Snippet: R8 cells were maintained in R10 (RPMI-1640 (Gibco), 10% FBS, 100 IU Penicillin and 100 μg/mL Streptomycin, and 55 μM 2-mercaptoethanol). .. For intracellular staining, cells were fixed with 4% Article Title: Targeted CRISPR screens reveal genes essential for Cryptosporidium survival in the host intestine Article Snippet: .. At 24 h post-infection, cell monolayers were washed with 1X PBS, fixed with 4% Article Title: Supporting Information Discovery of Orally Active Hydroxyethylamine Based SPPL2a Inhibitors Article Snippet: For all stable cell lines, cells were seeded at 3000 cells/30 μl/384well in DMEM / GlutaMaxTM-I (Invitrogen) supplemented with tetracycline-free 10% FBS (Amimed) and incubated at 37 °C, 5% S34 CO2 for 3-4 h. Subsequently, 3.3 μL of inhibitors, pre-diluted in doxycycline-containing medium for 11-point concentration response curves, were added to each well using a CyBi well liquid handling device (Cybio AG, Jena, Germany) to result in final inhibitor concentrations ranging from 100 μM to 1 nM (final DMSO concentration 0.9% (v/v)) and 5 μg/mL doxycycline). .. The cells were incubated with inhibitor at 37 °C, 5% CO2 for 24 h. Thereafter cell were fixed in 4% Negative Staining:Article Title: Tethered Exosomes Containing the Matrix Metalloproteinase MT1‐MMP Contribute to Extracellular Matrix Degradation Article Snippet: .. For sEV negative staining, EV samples were spotted on formvar/carbon‐coated copper/palladium grids for 20 min and fixed with 2% Transmission Assay:Article Title: Tethered Exosomes Containing the Matrix Metalloproteinase MT1‐MMP Contribute to Extracellular Matrix Degradation Article Snippet: .. For sEV negative staining, EV samples were spotted on formvar/carbon‐coated copper/palladium grids for 20 min and fixed with 2% Microscopy:Article Title: Tethered Exosomes Containing the Matrix Metalloproteinase MT1‐MMP Contribute to Extracellular Matrix Degradation Article Snippet: .. For sEV negative staining, EV samples were spotted on formvar/carbon‐coated copper/palladium grids for 20 min and fixed with 2% Incubation:Article Title: RSRC2 is a novel RNA-binding protein that safeguards mitotic fidelity by interacting with the lncRNA C1QTNF1-AS1. Article Snippet: .. Methanol fixation: slides were fixed in 99.9% ice-cold methanol (Acros Organics, 167830025) and incubated at −20 ◦C for 10 min. PFA fixation: slides were fixed in 4% Article Title: Supporting Information Discovery of Orally Active Hydroxyethylamine Based SPPL2a Inhibitors Article Snippet: For all stable cell lines, cells were seeded at 3000 cells/30 μl/384well in DMEM / GlutaMaxTM-I (Invitrogen) supplemented with tetracycline-free 10% FBS (Amimed) and incubated at 37 °C, 5% S34 CO2 for 3-4 h. Subsequently, 3.3 μL of inhibitors, pre-diluted in doxycycline-containing medium for 11-point concentration response curves, were added to each well using a CyBi well liquid handling device (Cybio AG, Jena, Germany) to result in final inhibitor concentrations ranging from 100 μM to 1 nM (final DMSO concentration 0.9% (v/v)) and 5 μg/mL doxycycline). .. The cells were incubated with inhibitor at 37 °C, 5% CO2 for 24 h. Thereafter cell were fixed in 4% |